Chromosome mapping using polymerase chain reaction on somatic cell hybrids

J Zhang; K Devriendt; P Marynen; H Van den Berghe; J J Cassiman

doi:10.1016/0165-4608(90)90085-o

Chromosome mapping using polymerase chain reaction on somatic cell hybrids

Cancer Genet Cytogenet. 1990 Apr;45(2):217-21. doi: 10.1016/0165-4608(90)90085-o.

Authors

J Zhang¹, K Devriendt, P Marynen, H Van den Berghe, J J Cassiman

Affiliation

¹ Center for Human Genetics, University of Leuven, Belgium.

PMID: 2317769
DOI: 10.1016/0165-4608(90)90085-o

Abstract

DNA polymerase chain reaction (PCR) was used to detect specific human sequences in human-mouse somatic hybrids. As compared with classic Southern blotting, this technique appeared to be more sensitive and less time-consuming for gene mapping.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Blotting, Southern
Cell Line
Chromosome Mapping*
Gene Amplification*
Humans
Hybrid Cells
Mice
Molecular Sequence Data
Polymerase Chain Reaction*
Pregnancy Proteins / genetics

Substances

Pregnancy Proteins