Alfentanil: correlations between absence of effect upon subcutaneous mast cells and absence of granuloma formation after intrathecal infusion in the dog

Neuromodulation. 2013 Sep-Oct;16(5):459-66; discussion 466. doi: 10.1111/j.1525-1403.2012.00534.x. Epub 2012 Nov 21.

Abstract

Background: We hypothesize that intrathecal (IT) granulomas arising from the IT infusion of several opiates may result from the degranulation of meningeal mast cells (MC). Given functional covariance between cutaneous and meningeal MC, we propose that opioids that do not degranulate cutaneous MC will not produce a granuloma. An opioid meeting this criteria is the phenylpiperadine alfentanil HCl.

Methods: Three experiments were accomplished in dogs. 1) Cutaneous MC degranulation. Flare areas on the dog abdomen were measured after intradermal alfentanil, morphine, or compound 48-80. 2) Dose ranging of analgesic effects of IT alfentanil infusion. Dogs with lumbar IT catheters received continuous infusion for 24 hours of different concentrations (1-20 mg/mL/d) of alfentanil and analgesic effects were assessed. 3) Granuloma inducing effects. Dogs received IT alfentanil (20 mg/mL/d; N = 5; 22-28 days) or morphine (12 mg/mL/d; N = 3; 22-30 days) and spinal cord harvested for histopathology after 22-30 days of infusion.

Results: 1) Intradermal morphine (10 mg/mL) and compound 48-80 (1 mg/mL) but not alfentanil at concentrations up to 20 mg/mL produced a cutaneous flare. IT alfentanil infusion produced increases in thermal escape latency at concentrations as low as 2 mg/mL/day. A significant depression of arousal was noted in the dogs receiving 20 mg/mL. Over the 22- to 30-day infusion period, morphine (12 mg/mL/day) resulted in granulomas in all three animals examined whereas IT alfentanil at 20 mg/mL/day failed to initiate a granuloma in any animal.

Conclusions: These results support the hypothesis linking MC degranulation and IT granulomas.

Keywords: Intraspinal drug delivery; intrathecal admixtures; intrathecal drug delivery; intrathecal granuloma; intrathecal pump; morphine; opioid.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alfentanil / administration & dosage*
  • Analgesics, Opioid / administration & dosage*
  • Analysis of Variance
  • Animals
  • Dogs
  • Dose-Response Relationship, Drug
  • Granuloma / chemically induced*
  • Injections, Spinal
  • Mast Cells / physiology*
  • Morphine / administration & dosage
  • Skin / pathology*

Substances

  • Analgesics, Opioid
  • Alfentanil
  • Morphine