Rational design of Hg2+ controlled streptavidin-binding aptamer

Chem Commun (Camb). 2013 Jan 7;49(2):164-6. doi: 10.1039/c2cc36416f. Epub 2012 Nov 20.

Abstract

Through substituting two canonical base pairs of a streptavidin-binding aptamer with T-T mismatched base pairs, a new aptamer was constructed. Its binding ability could be controlled by Hg(2+) through the formation of T-Hg-T metal-base pairs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / metabolism*
  • Base Pair Mismatch
  • Hemin / chemistry
  • Ions / chemistry
  • Mercury / chemistry*
  • Peroxidases / metabolism
  • Spectrometry, Fluorescence
  • Streptavidin / metabolism*
  • Thymidine / chemistry

Substances

  • Aptamers, Nucleotide
  • Ions
  • Hemin
  • Streptavidin
  • Peroxidases
  • Mercury
  • Thymidine