Abstract
In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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Cronobacter / classification*
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Cronobacter / enzymology
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Cronobacter / genetics*
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Cronobacter / isolation & purification
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Escherichia coli Proteins / genetics*
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Food Microbiology
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Foodborne Diseases / diagnosis
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Foodborne Diseases / microbiology
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Multiplex Polymerase Chain Reaction / methods*
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Phosphorus-Oxygen Lyases / genetics*
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Sensitivity and Specificity
Substances
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Escherichia coli Proteins
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Phosphorus-Oxygen Lyases
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diguanylate cyclase