Enhanced costimulation by CD70+ B cells aggravates experimental autoimmune encephalomyelitis in autoimmune mice

G Francosalinas; T Cantaert; M A Nolte; P P Tak; R A W van Lier; D L Baeten

doi:10.1016/j.jneuroim.2012.10.010

Enhanced costimulation by CD70+ B cells aggravates experimental autoimmune encephalomyelitis in autoimmune mice

J Neuroimmunol. 2013 Feb 15;255(1-2):8-17. doi: 10.1016/j.jneuroim.2012.10.010. Epub 2012 Nov 6.

Authors

G Francosalinas¹, T Cantaert, M A Nolte, P P Tak, R A W van Lier, D L Baeten

Affiliation

¹ Department of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

PMID: 23137837
DOI: 10.1016/j.jneuroim.2012.10.010

Abstract

Objective: Assess whether CD70+ B cells contribute to EAE.

Materials and methods: MOG-specific TCR transgenic mice (2D2) were crossed with mice with constitutive CD70 expression on B cells. The development of EAE and the phenotype of B-T lymphocytes were studied in 2D2xCD70 animals.

Results: Spontaneous EAE developed in 20% of 2D2xCD70 and 3% of 2D2 mice. EAE was also more severe in 2D2xCD70 versus 2D2 animals upon MOG immunization. The susceptibility of 2D2xCD70 to EAE was associated with fewer FoxP3+ T cells.

Conclusions: Expression of CD70 by B cells aggravates EAE possibly by reducing the number of regulatory T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
B-Lymphocyte Subsets / immunology*
B-Lymphocyte Subsets / metabolism
B-Lymphocyte Subsets / pathology*
CD27 Ligand / biosynthesis*
CD27 Ligand / genetics
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental / genetics
Encephalomyelitis, Autoimmune, Experimental / immunology*
Encephalomyelitis, Autoimmune, Experimental / pathology
Female
Immunophenotyping
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
T-Lymphocytes, Regulatory / immunology
T-Lymphocytes, Regulatory / metabolism
T-Lymphocytes, Regulatory / pathology

Substances

CD27 Ligand