[Effect of dexamethasone on expressions of TWEAK and Fn14 in the lung of asthmatic mice]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Nov;28(11):1138-41.
[Article in Chinese]

Abstract

Aim: To investigate the effect of dexamethasone (Dex) on the expressions of TNF-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor-inducible immediate-early response protein 14 (Fn14) in the lung of asthmatic mice.

Methods: Ovalbumin (OVA) was used to induce asthma in BALB/c mice. Thirty-six female mice were randomly divided into control group (n=12), asthmatic group (n=12) and Dex treated group (n=12). The airway inflammation was evaluated by HE staining. The expressions of TWEAK and Fn14 at mRNA and protein levels were detected by RT-PCR and immunohistochemistry, respectively.

Results: Both mRNA and protein levels of TWEAK and Fn14 in the asthmatic model group were significantly higher than those of control group (P<0.01), and both mRNA and protein levels of TWEAK and Fn14 in the Dex treated group were significantly lower than those of asthmatic group (P<0.01).

Conclusion: Dex can reduce the airway inflammation through inhibiting the expressions of TWEAK and Fn14.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asthma / drug therapy*
  • Asthma / metabolism
  • Cytokine TWEAK
  • Dexamethasone / pharmacology*
  • Dexamethasone / therapeutic use
  • Female
  • Lung / metabolism*
  • Lung / pathology
  • Mice
  • Mice, Inbred BALB C
  • RNA, Messenger / analysis
  • Receptors, Tumor Necrosis Factor / analysis
  • Receptors, Tumor Necrosis Factor / antagonists & inhibitors*
  • Receptors, Tumor Necrosis Factor / genetics
  • TWEAK Receptor
  • Tumor Necrosis Factor Inhibitors*
  • Tumor Necrosis Factors / analysis
  • Tumor Necrosis Factors / genetics

Substances

  • Cytokine TWEAK
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor
  • TWEAK Receptor
  • Tnfrsf12a protein, mouse
  • Tnfsf12 protein, mouse
  • Tumor Necrosis Factor Inhibitors
  • Tumor Necrosis Factors
  • Dexamethasone