Purification and amino-terminal sequence analysis of the complement-fixing and precipitin antigens from Coccidioides immitis

J Clin Microbiol. 1990 Feb;28(2):385-8. doi: 10.1128/jcm.28.2.385-388.1990.

Abstract

Two proteins (21 and 48 kilodaltons) purified from endospore-spherule culture filtrates of Coccidioides immitis are identified as precipitin and complement-fixing antigens, respectively. To allow specific structural comparison to antigens identified by other investigators and as a first step to eventual serodiagnostic antigen production by recombinant DNA technology, amino-terminal amino acid sequences were determined for these antigens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Fungal / isolation & purification*
  • Coccidioides / immunology*
  • Coccidioidomycosis / diagnosis
  • Coccidioidomycosis / immunology
  • Complement Fixation Tests
  • Fungal Proteins / immunology
  • Fungal Proteins / isolation & purification
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Precipitins / isolation & purification
  • Serologic Tests

Substances

  • Antigens, Fungal
  • Fungal Proteins
  • Precipitins