Single-round, multiplexed antibody mimetic design through mRNA display

Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12449-53. doi: 10.1002/anie.201207005. Epub 2012 Nov 4.

Abstract

In a single round: By combining the high-efficiency enrichment through the continuous-flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies / chemistry
  • Antibodies / metabolism
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Fibronectins / chemistry
  • Fibronectins / metabolism
  • Gene Library
  • Humans
  • Immunoglobulin Fc Fragments / chemistry
  • Immunoglobulin Fc Fragments / metabolism
  • Immunomagnetic Separation
  • Ligands
  • Maltose-Binding Proteins / chemistry
  • Maltose-Binding Proteins / metabolism
  • RNA, Messenger / chemistry*
  • RNA, Messenger / isolation & purification

Substances

  • Antibodies
  • Fibronectins
  • Immunoglobulin Fc Fragments
  • Ligands
  • Maltose-Binding Proteins
  • RNA, Messenger