Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections

J Clin Microbiol. 2013 Jan;51(1):295-8. doi: 10.1128/JCM.02221-12. Epub 2012 Oct 24.

Abstract

In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / genetics
  • Formaldehyde / metabolism
  • Humans
  • In Situ Hybridization / methods*
  • Oligonucleotide Probes* / genetics
  • Paraffin Embedding
  • Pathology, Molecular / methods*
  • Peptide Nucleic Acids* / genetics
  • Tissue Fixation
  • Trichosporon / genetics
  • Trichosporon / isolation & purification*
  • Trichosporonosis / diagnosis*

Substances

  • Oligonucleotide Probes
  • Peptide Nucleic Acids
  • Formaldehyde