Exonuclease-aided amplification for label-free and fluorescence turn-on DNA detection based on aggregation-induced quenching

Enguo Ju; Xinjian Yang; Youhui Lin; Fang Pu; Jinsong Ren; Xiaogang Qu

doi:10.1039/c2cc37039e

Exonuclease-aided amplification for label-free and fluorescence turn-on DNA detection based on aggregation-induced quenching

Chem Commun (Camb). 2012 Dec 11;48(95):11662-4. doi: 10.1039/c2cc37039e.

Authors

Enguo Ju¹, Xinjian Yang, Youhui Lin, Fang Pu, Jinsong Ren, Xiaogang Qu

Affiliation

¹ State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, China.

PMID: 23100143
DOI: 10.1039/c2cc37039e

Abstract

A facile, universal and label-free fluorescence turn-on amplification strategy for the detection of DNA has been reported, which can achieve ultralow femtomolar detection of a target gene sequence with high selectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pair Mismatch
DNA / analysis*
Exonucleases / metabolism*
Fluorescent Dyes / chemistry*
Imides / chemistry
Spectrometry, Fluorescence*
Tumor Suppressor Protein p53 / genetics

Substances

Fluorescent Dyes
Imides
Tumor Suppressor Protein p53
DNA
Exonucleases