Abstract
A novel phosphoramidite derivative of cholesterol, with an ether-linked hexaethylene glycol (HEG) spacer arm, has been obtained through simple and reproducible solid phase modified oligonucleotide synthesis manipulations. This building block and the known phosphoramidite derivative of 3b-(2-hydroxyethoxy)cholesterol have been exploited in standard oligonucleotide synthesis protocols for the preparation of 5'- conjugates of the G-quadruplex-forming ⁵'TGGGAG³' oligomer, known as the Hotoda's sequence, to produce new potential anti-HIV agents.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-HIV Agents / chemical synthesis*
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Anti-HIV Agents / chemistry
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Anti-HIV Agents / pharmacology*
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Base Sequence
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Cholesterol Esters / chemical synthesis*
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Cholesterol Esters / chemistry*
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Chromatography, High Pressure Liquid
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Ethylene Glycols / chemical synthesis*
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Ethylene Glycols / chemistry
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G-Quadruplexes*
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HIV / drug effects*
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Organophosphorus Compounds / chemical synthesis*
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Organophosphorus Compounds / chemistry
Substances
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Anti-HIV Agents
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Cholesterol Esters
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Ethylene Glycols
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Organophosphorus Compounds
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phosphoramidite
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hexaethylene glycol