Abstract
We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Calibration
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Lipid Bilayers / chemistry
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Membrane Proteins / chemistry*
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Metal Nanoparticles / chemistry*
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Nanotechnology / methods
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Protein Binding*
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Silicon Dioxide
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Silver / chemistry
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Solutions
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Spectrometry, Fluorescence / methods
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Spectrophotometry, Ultraviolet / methods
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Surface Plasmon Resonance
Substances
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Lipid Bilayers
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Membrane Proteins
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Solutions
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Silver
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Silicon Dioxide