Interaction of the echinocandin caspofungin with amphotericin B or voriconazole against Aspergillus biofilms in vitro

Antimicrob Agents Chemother. 2012 Dec;56(12):6414-6. doi: 10.1128/AAC.00687-12. Epub 2012 Oct 1.

Abstract

Aspergillus biofilms were prepared from 22 strains of Aspergillus spp. via a 96-well plate-based method. Using a broth microdilution checkerboard technique with the XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] colorimetric assay, we demonstrated a synergistic antifungal activity against 18 of 22 Aspergillus biofilm strains with a combination of caspofungin and amphotericin B and against 13 of 22 strains with a combination of caspofungin and voriconazole. We did not observe antagonism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphotericin B / pharmacology*
  • Antifungal Agents / pharmacology*
  • Aspergillus / drug effects*
  • Biofilms / drug effects*
  • Caspofungin
  • Colorimetry
  • Coloring Agents
  • Drug Synergism
  • Echinocandins / pharmacology*
  • Lipopeptides
  • Microbial Sensitivity Tests
  • Pyrimidines / pharmacology*
  • Tetrazolium Salts
  • Triazoles / pharmacology*
  • Voriconazole

Substances

  • Antifungal Agents
  • Coloring Agents
  • Echinocandins
  • Lipopeptides
  • Pyrimidines
  • Tetrazolium Salts
  • Triazoles
  • 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
  • Amphotericin B
  • Caspofungin
  • Voriconazole