Distinct developmental principles underlie the formation of ipsilateral and contralateral whisker-related axonal patterns of layer 2/3 neurons in the barrel cortex

Abstract

Axonal organizations with specific patterns underlie the functioning of local intracortical circuitry, but their precise anatomy and development still remain elusive. Here, we selectively visualized layer 2/3 neurons using in utero electroporation and examined their axonal organization in the barrel cortex contralateral to the electroporated side. We found that callosal axons run preferentially in septal regions of layer 4 and showed a whisker-related pattern in the contralateral barrel cortex in rats and mice. In addition, presynaptic marker proteins were found in this whisker-related axonal organization. Although the whisker-related patterns were observed in both the ipsilateral and contralateral barrel cortex, we found a difference in their developmental processes. While the formation of the whisker-related pattern in the ipsilateral cortex consisted of two distinct steps, that in the contralateral cortex did not have the 1st step, in which the axons were diffusely distributed without preference to septal or barrel regions. We also found that these more diffuse axons ran close to radial glial fibers. Together, our results uncovered a whisker-related axonal pattern of callosal axons and two independent developmental processes involved in the formation of the axonal trajectories of layer 2/3 neurons.