In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display

Biotechnol Lett. 2013 Jan;35(1):39-45. doi: 10.1007/s10529-012-1049-6. Epub 2012 Sep 18.

Abstract

A ribosome display from a diverse random library was applied for selecting peptide aptamers with high binding affinity to single-wall carbon nanotubes (SWCNTs). The selected peptide aptamer bound to and solubilized SWCNTs more strongly than did the peptide aptamer selected by a phage display method reported previously, and more strongly than other commonly used organic surfactants. The fluorescence spectrum of this aptamer showed a red shift upon interaction with SWCNTs but circular dichroism spectroscopy did not show any significant difference between the presence or absence of SWCNT binding.

MeSH terms

  • Amino Acid Sequence
  • Aptamers, Peptide / chemistry
  • Aptamers, Peptide / genetics
  • Aptamers, Peptide / metabolism*
  • Biotechnology / methods*
  • Molecular Sequence Data
  • Nanotubes, Carbon / chemistry*
  • Peptide Library*
  • Protein Binding
  • Ribosomes / chemistry
  • Ribosomes / genetics
  • Ribosomes / metabolism*
  • Spectrum Analysis

Substances

  • Aptamers, Peptide
  • Nanotubes, Carbon
  • Peptide Library