Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films

Yukiko Shinozaki; Takashi Watanabe; Toshiaki Nakajima-Kambe; Hiroko K Kitamoto

doi:10.1016/j.jbiosc.2012.08.010

Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films

J Biosci Bioeng. 2013 Jan;115(1):111-4. doi: 10.1016/j.jbiosc.2012.08.010. Epub 2012 Sep 14.

Authors

Yukiko Shinozaki¹, Takashi Watanabe, Toshiaki Nakajima-Kambe, Hiroko K Kitamoto

Affiliation

¹ National Institute for Agro-environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.

PMID: 22986307
DOI: 10.1016/j.jbiosc.2012.08.010

Abstract

We developed a rapid and simple method for evaluating the degradation of solid biodegradable plastics (BPs). Dye-containing BP films were used as substrates and the release of dye caused by the degradation of BPs was confirmed by a color change in the enzyme solution after a reaction time of 24 h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradable Plastics / analysis*
Biodegradable Plastics / metabolism*
Color
Colorimetry / methods*
Coloring Agents / analysis
Enzyme Assays
Enzymes / metabolism*
Time Factors

Substances

Biodegradable Plastics
Coloring Agents
Enzymes