The analysis of the polar fraction of Galeopsis angustifolia Ehrh. ex Hoffm. indicates that the main components are iridoids and flavonoids. Six compounds were identified: a new flavonoid, 3'-hydroxy-isoscutellarein 7-O-[6'″acetyl-β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranoside]; three iridoid glucosides: harpagide, acetyl harpagide and for the first time in this species, 8-epi-loganin; two known acetylated flavonoid glycosides: 3'-hydroxy-4'-O-methylisoscutellarein 7-O-[6'″acetyl-β-D-allopyranosyl-(1 → 2)-6″-acetyl-β-D-glucopyranoside], 3'-hydroxy-4'-O-methylisoscutellarein 7-O-[6'″acetyl-β-D-allopyranosyl-(1 → 2)-β-D-glucopyranoside]. Both flavonoids and iridoids are present in high amount; respectively, 16.7% and 4.5% of the crude extract.