Comparison of Tear cytokines and clinical outcomes between off-flap and on-flap epi-LASIK with mitomycin C

J Refract Surg. 2012 Sep;28(9):632-8. doi: 10.3928/1081597X-20120815-01.

Abstract

Purpose: To compare tear cytokines and clinical outcomes between off-flap and on-flap epi-LASIK eyes and explore the possible mechanism for the clinical differences.

Methods: This double-masked, randomized study enrolled 18 myopic patients who underwent off-flap epi-LASIK with mitomycin C (MMC) in 1 eye and on-flap epi-LASIK with MMC in the contralateral eye. Tears were collected from each eye preoperatively and 2 hours, 1 day, and 5 days postoperatively. Concentrations of multiple tear cytokines were measured by a multiplex immunobead assay. Uncorrected distance visual acuity (UDVA), refraction, haze scores, pain scores, and percentage of corneal epithelial healing were evaluated.

Results: Compared with the on-flap group, the off-flap group had outcomes of better UDVA and higher percentages of epithelial healing at 5 days after surgery (P<.001) and lower levels of haze at 1 month after surgery (P=.049). Preoperatively, no significant differences were noted in the release rate of all tear cytokines between groups. At 2 hours postoperatively, the release rate of tear basic fibroblast growth factor (bFGF), platelet-derived growth factor-BB (PDGF-BB), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-α) in the off-flap group were significantly lower than those in the on-flap group (P=.011, .017, .048, and .041, respectively).

Conclusions: Off-flap epi-LASIK with MMC offers faster corneal epithelial healing and visual recovery, and temporary less haze than on-flap epi-LASIK with MMC. The lower tear levels of bFGF, PDGF-BB, IL-8, and TNF-α in the offflap group 2 hours after surgery may suggest a possible mechanism for the clinical differences.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Alkylating Agents / administration & dosage*
  • Combined Modality Therapy
  • Cytokines / metabolism*
  • Double-Blind Method
  • Eye Pain / physiopathology
  • Eye Proteins / metabolism
  • Female
  • Follow-Up Studies
  • Humans
  • Immunoassay
  • Keratomileusis, Laser In Situ / methods*
  • Lasers, Excimer / therapeutic use*
  • Male
  • Mitomycin / administration & dosage*
  • Myopia / physiopathology
  • Myopia / surgery
  • Pain Measurement
  • Refraction, Ocular / physiology
  • Surgical Flaps*
  • Tears / metabolism*
  • Treatment Outcome
  • Visual Acuity / physiology
  • Wound Healing / physiology
  • Young Adult

Substances

  • Alkylating Agents
  • Cytokines
  • Eye Proteins
  • tear proteins
  • Mitomycin