A computational framework for boosting confidence in high-throughput protein-protein interaction datasets

Genome Biol. 2012 Aug 31;13(8):R76. doi: 10.1186/gb-2012-13-8-r76.

Abstract

Improving the quality and coverage of the protein interactome is of tantamount importance for biomedical research, particularly given the various sources of uncertainty in high-throughput techniques. We introduce a structure-based framework, Coev2Net, for computing a single confidence score that addresses both false-positive and false-negative rates. Coev2Net is easily applied to thousands of binary protein interactions and has superior predictive performance over existing methods. We experimentally validate selected high-confidence predictions in the human MAPK network and show that predicted interfaces are enriched for cancer -related or damaging SNPs. Coev2Net can be downloaded at http://struct2net.csail.mit.edu.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Computational Biology / methods*
  • Databases, Protein*
  • High-Throughput Screening Assays
  • Humans
  • Mitogen-Activated Protein Kinases / genetics
  • Mitogen-Activated Protein Kinases / metabolism
  • Mutation, Missense
  • Polymorphism, Single Nucleotide
  • Protein Conformation
  • Protein Interaction Mapping / methods*
  • Reproducibility of Results
  • Software

Substances

  • Mitogen-Activated Protein Kinases