Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy

Alon Greenbaum; Wei Luo; Ting-Wei Su; Zoltán Göröcs; Liang Xue; Serhan O Isikman; Ahmet F Coskun; Onur Mudanyali; Aydogan Ozcan

doi:10.1038/nmeth.2114

Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy

Nat Methods. 2012 Sep;9(9):889-95. doi: 10.1038/nmeth.2114. Epub 2012 Aug 30.

Authors

Alon Greenbaum¹, Wei Luo, Ting-Wei Su, Zoltán Göröcs, Liang Xue, Serhan O Isikman, Ahmet F Coskun, Onur Mudanyali, Aydogan Ozcan

Affiliation

¹ Electrical Engineering Department, University of California, Los Angeles, Los Angeles, California, USA.

Abstract

We discuss unique features of lens-free computational imaging tools and report some of their emerging results for wide-field on-chip microscopy, such as the achievement of a numerical aperture (NA) of ∼0.8-0.9 across a field of view (FOV) of more than 20 mm(2) or an NA of ∼0.1 across a FOV of ∼18 cm(2), which corresponds to an image with more than 1.5 gigapixels. We also discuss the current challenges that these computational on-chip microscopes face, shedding light on their future directions and applications.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Erythrocytes / cytology
Humans
Image Processing, Computer-Assisted / instrumentation*
Image Processing, Computer-Assisted / methods*
Lenses
Male
Microscopy / instrumentation*
Microscopy / methods*
Spermatozoa / cytology

Abstract

Publication types

MeSH terms

Grants and funding