Abstract
Contaminated vaccine is one unexpected and potential origin of virus infection. In order to investigate the most likely cause of disease in a broiler breeder company of Shandong Province, all 17 batches of live-virus vaccines used in the affected flocks and 478 tissue samples were tested by dot-blot hybridization, nested PCR, and IFA. The results suggested the outbreak of disease was most probably due to the vaccination of REV-contaminated MD-CVI988/Rispens vaccines and ND-LaSota+IB-H120 vaccines. Furthermore, the REV was probably transmitted to the commercial chickens through congenital transmission.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / immunology
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Cells, Cultured
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Chick Embryo / cytology
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Chickens / immunology*
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Chickens / virology
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DNA, Viral / genetics
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Drug Contamination
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Female
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Fibroblasts / immunology
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Fibroblasts / virology
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Fluorescent Antibody Technique, Indirect
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Gene Products, env / genetics
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Male
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Nucleic Acid Hybridization
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Polymerase Chain Reaction
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Poultry Diseases / immunology*
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Poultry Diseases / transmission
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Poultry Diseases / virology
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Reticuloendotheliosis virus / genetics
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Reticuloendotheliosis virus / immunology*
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Spleen / virology
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Vaccination
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Viral Vaccines / administration & dosage
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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DNA, Viral
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Gene Products, env
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Viral Vaccines
Grants and funding
This work was supported by National Natural Science Foundation of China (30972183, 30740077), Special Fund Research of Public Welfare Industries of Ministry of Agriculture (200803019), and Science and Technology Development Plan of Shandong Province (2012GNC11020). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.