Abstract
Purpose:
To investigate the influence of lactate on expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in rat retina.
Methods:
Retinal explants from neonatal Sprague Dawley rats were incubated with media containing 10, 20, or 30 mM of lactic acid. The 10 mM group was used as a control. At 24 h after incubation, retinas were sectioned for light microscopy, and expressions of VEGF and PEDF measured by real-time polymerase chain reaction (RT-PCR) and Western blot analysis.
Results:
The architecture of cultured retinas appeared to be intact. Compared with control, both RT-PCR and Western blot analysis showed that 30 mM of lactic acid significantly increased the levels of VEGF, but not PEDF.
Conclusions:
Stimulation of production of retinal VEGF by lactate is dependent on the concentration of lactate. Lactate has no effect on the expression of PEDF in rat retinal explants.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Blotting, Western
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DNA / genetics*
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Eye Proteins / biosynthesis
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Eye Proteins / drug effects
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Eye Proteins / genetics*
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Gene Expression Regulation, Developmental / drug effects*
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Lactic Acid / pharmacology*
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Male
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Nerve Growth Factors / biosynthesis
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Nerve Growth Factors / drug effects
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Nerve Growth Factors / genetics*
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Rats
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Rats, Sprague-Dawley
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Retina / drug effects
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Retina / growth & development
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Retina / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Serpins / biosynthesis
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Serpins / drug effects
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Serpins / genetics*
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Tissue Culture Techniques
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Vascular Endothelial Growth Factor A / biosynthesis
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Vascular Endothelial Growth Factor A / drug effects
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Vascular Endothelial Growth Factor A / genetics*
Substances
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Eye Proteins
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Nerve Growth Factors
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Serpins
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Vascular Endothelial Growth Factor A
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pigment epithelium-derived factor
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Lactic Acid
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DNA