Flow cytometric analysis of DNA ploidy in large cleaved cell lymphomas

Hematol Oncol. 1990 Nov-Dec;8(6):339-46. doi: 10.1002/hon.2900080606.

Abstract

The results of nuclear DNA content analysis of a series of 28 patients with large cleaved follicular centre cell non-Hodgkin's lymphomas (NHL) are reported. DNA aneuploidy was found in 11 (39 per cent) cases. The DNA indices of the DNA-aneuploid peaks ranged from 1.14 to 2.28. Seven (25 per cent) cases were tetraploid. DNA ploidy was not associated with prognosis. The percentage of S phase cells (SPF) ranged from 2.0 to 30.5 per cent (median 5.1 per cent). Lymphoma patients with SPF higher than 9.7 per cent had a worse survival rate than patients with lymphoma with less than 9.7 per cent S phase cells but the difference did not reach statistical significance. The results of the DNA ploidy and SPF were comparable to those of intermediate and high grade malignancy NHLs.

MeSH terms

  • Adult
  • Aged
  • Aneuploidy
  • DNA, Neoplasm / genetics*
  • Female
  • Flow Cytometry
  • Humans
  • Lymphoma, B-Cell / classification
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / pathology
  • Lymphoma, Follicular / classification
  • Lymphoma, Follicular / genetics*
  • Lymphoma, Follicular / pathology
  • Male
  • Middle Aged
  • Ploidies*
  • Prognosis
  • S Phase

Substances

  • DNA, Neoplasm