High-performance liquid chromatography of globin chains in the identification of human globin gene abnormalities

Biophys Chem. 1990 Aug 31;37(1-3):225-30. doi: 10.1016/0301-4622(90)88021-j.

Abstract

This article summarizes experience and data obtained using a previously developed reverse-phase high-performance liquid chromatography method (J.B. Shelton, J.R. Shelton and W.A. Schroeder, J. Liq. Chromatogr. 7 (1984) 1969) in the study of a number of hemoglobinopathies in the Sardinian population. The occurrence and incidence of several abnormal hemoglobins are described, as well as aspects of the expression of abnormal gamma-globin gene arrangements and thalassemic genes.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Chromosome Deletion
  • Gene Rearrangement
  • Genes*
  • Genetic Variation
  • Globins / genetics*
  • Globins / isolation & purification
  • Hemoglobin A / genetics*
  • Humans
  • Infant, Newborn
  • Macromolecular Substances
  • Mutation
  • Reference Values

Substances

  • Macromolecular Substances
  • Globins
  • Hemoglobin A