Abstract
The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Carbocyanines / analysis
-
Carbocyanines / metabolism
-
Cell Survival
-
Escherichia coli / enzymology*
-
Escherichia coli / genetics
-
Fluorescent Dyes / analysis
-
Fluorescent Dyes / metabolism*
-
HEK293 Cells
-
Histones / analysis
-
Histones / genetics
-
Histones / metabolism
-
Humans
-
Microscopy, Fluorescence / methods*
-
Recombinant Fusion Proteins / analysis
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Tetrahydrofolate Dehydrogenase / analysis
-
Tetrahydrofolate Dehydrogenase / genetics*
-
Tetrahydrofolate Dehydrogenase / metabolism
-
Transfection
-
Trimethoprim / analysis
-
Trimethoprim / metabolism*
-
Viscosity
Substances
-
Carbocyanines
-
Fluorescent Dyes
-
Histones
-
Recombinant Fusion Proteins
-
cyanine dye 3
-
Trimethoprim
-
Tetrahydrofolate Dehydrogenase