Evaluation of Clearview Exact PBP2a, a new immunochromatographic assay, for detection of low-level methicillin-resistant Staphylococcus aureus (LL-MRSA)

J Clin Microbiol. 2012 Oct;50(10):3359-60. doi: 10.1128/JCM.01829-12. Epub 2012 Jul 18.

Abstract

We evaluated the performance of a new immunochromatographic assay (ICA), the Clearview Exact PBP2a, for rapid detection of penicillin-binding protein 2a (PBP2a) in a challenge set of Staphylococcus aureus strains showing MICs to oxacillin of ≤16 mg/liter. The sensitivity and specificity of the ICA were 96.6% and 100%, respectively.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteriological Techniques / methods*
  • Chromatography, Affinity / methods*
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Microbial Sensitivity Tests
  • Oxacillin / pharmacology
  • Penicillin-Binding Proteins / analysis*
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*

Substances

  • Anti-Bacterial Agents
  • Penicillin-Binding Proteins
  • Oxacillin