Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase

Chembiochem. 2012 Jul 23;13(11):1559-63. doi: 10.1002/cbic.201200256. Epub 2012 Jun 22.

Abstract

Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocatalysis
  • Fluorescence
  • Fluorides / analysis*
  • Humans
  • Hydrocarbons, Fluorinated / chemistry
  • Hydrocarbons, Fluorinated / metabolism
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Protons
  • gamma-Butyrobetaine Dioxygenase / analysis*
  • gamma-Butyrobetaine Dioxygenase / metabolism*

Substances

  • Hydrocarbons, Fluorinated
  • Protons
  • gamma-Butyrobetaine Dioxygenase
  • Fluorides