Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

Takashi Fujikawa; Toru Iwanami

doi:10.1016/j.mcp.2012.06.001

Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

Mol Cell Probes. 2012 Oct;26(5):194-7. doi: 10.1016/j.mcp.2012.06.001. Epub 2012 Jun 21.

Authors

Takashi Fujikawa¹, Toru Iwanami

Affiliation

¹ National Institute of Fruit Tree Science, Fujimoto 2-1, Tsukuba, Ibaraki 305-8605, Japan.

PMID: 22728344
DOI: 10.1016/j.mcp.2012.06.001

Abstract

Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with our primers than with the common primers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Citrus / microbiology*
DNA Primers / genetics*
DNA, Ribosomal
Japan
Plant Diseases / microbiology
Plant Leaves / microbiology
Polymerase Chain Reaction / methods*
Rhizobiaceae / genetics*
Rhizobiaceae / pathogenicity
Sensitivity and Specificity

Substances

DNA Primers
DNA, Ribosomal