An activity-based fluorogenic probe for sensitive and selective monoamine oxidase-B detection

Shaobo Long; Lin Chen; Yumi Xiang; Minggui Song; Yuguo Zheng; Qing Zhu

doi:10.1039/c2cc33089j

An activity-based fluorogenic probe for sensitive and selective monoamine oxidase-B detection

Chem Commun (Camb). 2012 Jul 21;48(57):7164-6. doi: 10.1039/c2cc33089j. Epub 2012 Jun 12.

Authors

Shaobo Long¹, Lin Chen, Yumi Xiang, Minggui Song, Yuguo Zheng, Qing Zhu

Affiliation

¹ Institute of Bioengineering, Zhejiang University of Technology, Chaowang Road 18, China.

PMID: 22692558
DOI: 10.1039/c2cc33089j

Abstract

We report the design and synthesis of a new class of fluorogenic probes based on monoamine oxidase-triggered oxidative C-O bond cleavage. The selectivity of probe P1 towards MAO-B was 22-fold higher than that towards MAO-A.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Coumarins / chemistry*
Coumarins / metabolism
Enzyme Assays / methods*
Fluorescent Dyes / chemistry*
Fluorescent Dyes / metabolism
Humans
Models, Molecular
Monoamine Oxidase / analysis
Monoamine Oxidase / metabolism*
Oxidation-Reduction
Protein Binding
Sensitivity and Specificity
Spectrometry, Fluorescence / methods

Substances

Coumarins
Fluorescent Dyes
Monoamine Oxidase