Abstract
In this study, we conducted a survey of the cytochrome b (cytb) gene of Babesia gibsoni (B. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (ATV)-resistant variants. Ninety-two blood samples were collected from naturally B. gibsoni infected dogs. The cytb nucleotide sequence was determined by direct sequencing. Twelve non-synonymous amino acid substitutions were identified in cytb. The principal ATV-resistant substitution, M121I, was detected in three cases. This survey determined that potentially ATV-resistant B. gibsoni strains are present in dogs in Japan.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Atovaquone / therapeutic use
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Babesia / enzymology
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Babesia / genetics
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Babesia / isolation & purification*
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Babesiosis / drug therapy
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Babesiosis / epidemiology
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Babesiosis / parasitology
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Babesiosis / veterinary*
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Cytochromes b / genetics*
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DNA, Protozoan / chemistry
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DNA, Protozoan / genetics
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Dog Diseases / drug therapy
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Dog Diseases / epidemiology*
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Dog Diseases / parasitology*
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Dogs
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Enzyme Inhibitors / therapeutic use
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Japan / epidemiology
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Point Mutation
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Polymerase Chain Reaction / veterinary
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RNA, Ribosomal, 18S / chemistry
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RNA, Ribosomal, 18S / genetics
Substances
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DNA, Protozoan
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Enzyme Inhibitors
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RNA, Ribosomal, 18S
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Cytochromes b
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Atovaquone