Immunolocalization of bone morphogenetic protein 2 during the early healing events after guided bone regeneration

Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Apr;113(4):533-41. doi: 10.1016/j.oooo.2011.09.016.

Abstract

Objective: The objective of this study was to evaluate the immunolocalization of bone morphogenetic protein 2 (BMP-2) after autogenous block grafting covered or not with an e-PTFE membrane.

Study design: Forty-eight rats were divided into 2 groups, autogenous block graft (B) and autogenous block graft + e-PTFE membrane (MB), and were evaluated by immunohistochemistry at baseline and 3, 7, 14, 21, and 45 days.

Results: The largest number of positive cells in the recipient bed was observed after 3 days in both groups. At the graft border, the largest number of positive cells was seen after 7 days in group B and after 14 days in group MB. The highest proportion of staining in the graft was observed after 3 days in group B and after 21 days in group MB.

Conclusions: High proportions of stain were related to intense revascularization and osteogenesis. Except for the interface, BMP-2 staining occurred later in group MB than in group B in all structures analyzed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 2 / biosynthesis*
  • Bone Morphogenetic Protein 2 / physiology
  • Bone Regeneration*
  • Bone Transplantation
  • Guided Tissue Regeneration, Periodontal / methods*
  • Male
  • Mandible / blood supply
  • Mandible / surgery
  • Membranes, Artificial
  • Neovascularization, Physiologic*
  • Osteoblasts / metabolism
  • Osteocytes / metabolism
  • Polytetrafluoroethylene
  • Rats
  • Rats, Wistar
  • Stem Cells / metabolism

Substances

  • Bone Morphogenetic Protein 2
  • Membranes, Artificial
  • Polytetrafluoroethylene