Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa

Tatsuya Tada; Tohru Miyoshi-Akiyama; Masashi Tanaka; Kenji Narahara; Masahiro Shimojima; Tomoe Kitao; Kayo Shimada; Teruo Kirikae

doi:10.1016/j.mimet.2012.05.009

Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa

J Microbiol Methods. 2012 Oct;91(1):114-6. doi: 10.1016/j.mimet.2012.05.009. Epub 2012 May 20.

Authors

Tatsuya Tada¹, Tohru Miyoshi-Akiyama, Masashi Tanaka, Kenji Narahara, Masahiro Shimojima, Tomoe Kitao, Kayo Shimada, Teruo Kirikae

Affiliation

¹ Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.

PMID: 22617868
DOI: 10.1016/j.mimet.2012.05.009

Abstract

To detect aminoglycoside 6'-N-acetyltransferase-Ib [AAC(6')-Ib]-producing, Pseudomonas aeruginosa isolates which are a frequent cause of nosocomial infections in Japan, an immunochromatographic assay was developed using two kinds of monoclonal antibodies (mAbs) recognizing AAC(6')-Ib. The results of the assessment were fully consistent with those of aac(6')-Ib PCR analyses.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetyltransferases / analysis*
Antibodies, Bacterial
Antibodies, Monoclonal
Chromatography, Affinity / methods*
Humans
Japan
Pseudomonas aeruginosa / enzymology*

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
Acetyltransferases
aminoglycoside N(6')-acetyltransferase