Abstract
Homogenous Mn-peroxidase of a 26-fold purity grade was isolated from a culture of Azospirillum brasilense Sp245 cultivated on a medium containing 0.1 mM pyrocatechol. The molecular weight of the enzyme is 43 kD as revealed by electrophoresis in SDS-PAAG. It was shown that the use of pyrocatechol and 2,2'-azino-bis(3-ethylbenzotiazoline-6-sulfonate) at concentrations of 0.1 and I mM as inductors increased the Mn-peroxidase activity by a factor of 3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azospirillum brasilense* / drug effects
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Azospirillum brasilense* / enzymology
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Azospirillum brasilense* / isolation & purification
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / isolation & purification
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Benzothiazoles / pharmacology
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Catechols / pharmacology
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Culture Media
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Electrophoresis, Polyacrylamide Gel
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Enzyme Induction
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Kinetics
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Molecular Weight
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Peroxidases / biosynthesis*
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Peroxidases / isolation & purification
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Soil Microbiology*
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Sulfonic Acids / pharmacology
Substances
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Bacterial Proteins
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Benzothiazoles
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Catechols
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Culture Media
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Sulfonic Acids
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Peroxidases
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manganese peroxidase
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catechol