A novel partial sequence alignment tool for finding large deletions

ScientificWorldJournal. 2012:2012:694813. doi: 10.1100/2012/694813. Epub 2012 Apr 1.

Abstract

Finding large deletions in genome sequences has become increasingly more useful in bioinformatics, such as in clinical research and diagnosis. Although there are a number of publically available next generation sequencing mapping and sequence alignment programs, these software packages do not correctly align fragments containing deletions larger than one kb. We present a fast alignment software package, BinaryPartialAlign, that can be used by wet lab scientists to find long structural variations in their experiments. For BinaryPartialAlign, we make use of the Smith-Waterman (SW) algorithm with a binary-search-based approach for alignment with large gaps that we called partial alignment. BinaryPartialAlign implementation is compared with other straight-forward applications of SW. Simulation results on mtDNA fragments demonstrate the effectiveness (runtime and accuracy) of the proposed method.

MeSH terms

  • Algorithms*
  • Base Sequence
  • Computational Biology / methods
  • Computer Simulation
  • DNA, Mitochondrial / genetics*
  • Internet
  • Mitochondria / genetics
  • Reproducibility of Results
  • Sequence Alignment / methods*
  • Sequence Analysis, DNA / methods
  • Sequence Deletion*
  • Software*

Substances

  • DNA, Mitochondrial