In vitro chemoenzymatic and in vivo biocatalytic syntheses of new beauvericin analogues

Chem Commun (Camb). 2012 Jun 7;48(45):5674-6. doi: 10.1039/c2cc31669b. Epub 2012 May 1.

Abstract

New beauvericins have been synthesized using the nonribosomal peptide synthetase BbBEAS from the entomopathogenic fungus Beauveria bassiana. Chemical diversity was generated by in vitro chemoenzymatic and in vivo whole cell biocatalytic syntheses using either a B. bassiana mutant or an E. coli strain expressing the bbBeas gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beauveria / chemistry
  • Beauveria / enzymology*
  • Beauveria / genetics
  • Biocatalysis
  • Cloning, Molecular
  • Depsipeptides / chemistry*
  • Depsipeptides / metabolism*
  • Escherichia coli / genetics
  • Mutation
  • Peptide Synthases / genetics
  • Peptide Synthases / metabolism*

Substances

  • Depsipeptides
  • beauvericin
  • Peptide Synthases