Protonated triplex DNA in E. coli cells as detected by chemical probing

P Karlovsky; P Pecinka; M Vojtiskova; E Makaturova; E Palecek

doi:10.1016/0014-5793(90)81324-h

Protonated triplex DNA in E. coli cells as detected by chemical probing

FEBS Lett. 1990 Nov 12;274(1-2):39-42. doi: 10.1016/0014-5793(90)81324-h.

Authors

P Karlovsky¹, P Pecinka, M Vojtiskova, E Makaturova, E Palecek

Affiliation

¹ Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.

PMID: 2253780
DOI: 10.1016/0014-5793(90)81324-h

Abstract

The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro.

MeSH terms

Base Sequence
Binding Sites
DNA, Bacterial / chemistry*
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Escherichia coli / genetics*
Macromolecular Substances
Molecular Sequence Data
Nucleic Acid Conformation
Osmium
Plasmids
Restriction Mapping

Substances

DNA, Bacterial
Macromolecular Substances
Osmium