Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells

Opt Express. 2012 Apr 23;20(9):9673-81. doi: 10.1364/OE.20.009673.

Abstract

We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wavelength-dependent quantitative phase images in a single shot of a color-coded hologram. Using molecular specific dispersion, we demonstrate the extraction of Hb concentration and the dynamic membrane fluctuation from individual RBCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Colorimetry / instrumentation
  • Equipment Design
  • Equipment Failure Analysis
  • Erythrocyte Membrane / physiology*
  • Erythrocyte Membrane / ultrastructure*
  • Hemoglobins / analysis*
  • Holography / instrumentation
  • Humans
  • Membrane Fluidity*
  • Microscopy, Phase-Contrast / instrumentation*
  • Molecular Imaging / instrumentation*
  • Spectrum Analysis / instrumentation*

Substances

  • Hemoglobins