Conjugated polyelectrolyte-antibody hybrid materials for highly fluorescent live cell-imaging

Adv Mater. 2012 May 8;24(18):2479-84. doi: 10.1002/adma.201103895. Epub 2012 Apr 10.

Abstract

Conjugated polyelectrolyte-antibody hybrid materials promise to enhance the utility of conjugated polymers in bioimaging field. Polymer-antibody conjugates that are biologically safe and highly sensitive and selective to cells are designed to image human T or B lymphocytes. In the clear state, the observed efficiency of luminescence is superior to that of commercially available FITC-antibody probe.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / chemistry*
  • Antibodies / immunology
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology
  • Cell Line
  • Cell Survival / drug effects
  • Electrolytes / chemistry*
  • Fluorescein-5-isothiocyanate / chemistry*
  • Humans
  • Jurkat Cells
  • Microscopy, Confocal
  • Polymers / chemistry
  • Polymers / toxicity
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology

Substances

  • Antibodies
  • Electrolytes
  • Polymers
  • poly(phenylene ethynylene)
  • Fluorescein-5-isothiocyanate