Abstract
We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Carbapenems / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics*
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Real-Time Polymerase Chain Reaction / methods*
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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beta-Lactamases
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carbapenemase