Abstract
We presented a novel platform for an ultrafast PCR system, called the Rotary PCR Genetic Analyzer, which incorporates a thermal block and resistive temperature detector (RTD) for thermal cycling control, a disposable PCR microchip, and a stepper motor. The influenza viral RNAs from H3N2, H5N1, and H1N1 were simultaneously identified with high sensitivity and speed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Equipment Design
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Influenza A virus / classification
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Influenza A virus / genetics
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Influenza A virus / isolation & purification*
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Lab-On-A-Chip Devices
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RNA, Viral / analysis*
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Reverse Transcriptase Polymerase Chain Reaction / instrumentation*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Time Factors
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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RNA, Viral