A universal expression tag for structural and functional studies of proteins

Chembiochem. 2012 May 7;13(7):959-63. doi: 10.1002/cbic.201200045. Epub 2012 Mar 20.

Abstract

Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Expressed Sequence Tags / chemistry*
  • Genetic Vectors / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Proteins / chemistry*
  • Proteins / genetics
  • Recombinant Fusion Proteins / biosynthesis*
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Ubiquitin / genetics
  • Ubiquitin / metabolism

Substances

  • Proteins
  • Recombinant Fusion Proteins
  • Ubiquitin