Cutting edge: protein phosphatase 2A confers susceptibility to autoimmune disease through an IL-17-dependent mechanism

J Immunol. 2012 Apr 15;188(8):3567-71. doi: 10.4049/jimmunol.1200143. Epub 2012 Mar 14.

Abstract

The contribution of individual molecular aberrations to the pathogenesis of systemic lupus erythematosus (SLE), an autoimmune disease that affects multiple organs, is often difficult to evaluate because of the presence of abundant confounding factors. To assess the effect of increased expression of the phosphatase protein phosphatase 2A (PP2A) in T cells, as recorded in SLE patients, we generated a transgenic mouse that overexpresses the PP2Ac subunit in T cells. The transgenic mouse displays a heightened susceptibility to immune-mediated glomerulonephritis in the absence of other immune defects. CD4(+) T cells produce increased amounts of IL-17 while the number of neutrophils in the peripheral blood is increased. IL-17 neutralization abrogated the development of glomerulonephritis. We conclude that increased PP2Ac expression participates in SLE pathogenesis by promoting inflammation through unchecked IL-17 production and facilitating the development of end-organ damage.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / pharmacology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • Disease Models, Animal
  • Disease Susceptibility
  • Gene Expression / immunology*
  • Glomerulonephritis / chemically induced
  • Glomerulonephritis / immunology*
  • Humans
  • Interleukin-17 / biosynthesis
  • Interleukin-17 / immunology*
  • Lupus Erythematosus, Systemic / genetics
  • Lupus Erythematosus, Systemic / immunology*
  • Lupus Erythematosus, Systemic / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Protein Phosphatase 2 / genetics
  • Protein Phosphatase 2 / immunology*
  • Protein Phosphatase 2 / metabolism
  • Protein Subunits / genetics
  • Protein Subunits / immunology
  • Protein Subunits / metabolism

Substances

  • Antibodies, Neutralizing
  • Interleukin-17
  • Protein Subunits
  • Protein Phosphatase 2