A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

D Hsu; L M Shih; A E Castro; Y C Zee

doi:10.1007/BF01310755

A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

Arch Virol. 1990;114(3-4):259-63. doi: 10.1007/BF01310755.

Authors

D Hsu¹, L M Shih, A E Castro, Y C Zee

Affiliation

¹ Department of Veterinary Microbiology and Immunology, University of California, Davis.

PMID: 2241575
DOI: 10.1007/BF01310755

Abstract

A sensitive diagnostic method specific for alcelaphine herpesvirus-1, causative agent of malignant catarrhal fever, has been developed. Based on the nucleotide sequence of the alcelaphine herpesvirus-1 genomic DNA, a pair of 30 nucleotide primers was selected and synthesized for detecting the virus genome using the polymerase chain reaction (PCR). The virus genome was detected in crude cell lysate using the amplification reaction.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Base Sequence
DNA, Viral / analysis
Herpesviridae / genetics
Herpesviridae / isolation & purification*
Molecular Sequence Data
Polymerase Chain Reaction*
Vero Cells

Substances

DNA, Viral