Abstract
General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, Liquid
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DNA Helicases / metabolism*
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Gene Deletion
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Gene Expression Regulation, Fungal / radiation effects
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Humans
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Mass Spectrometry
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Phenotype
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Phosphorylation / radiation effects
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Protein Binding / radiation effects
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Protein Subunits / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / radiation effects
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / metabolism*
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Temperature
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Transcription Factor TFIIH / chemistry
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Transcription Factor TFIIH / metabolism*
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
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Transcription, Genetic* / radiation effects
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Ultraviolet Rays
Substances
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Protein Subunits
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RNA, Messenger
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SSL2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Tfb6 protein, S cerevisiae
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Transcription Factors
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Transcription Factor TFIIH
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DNA Helicases