A portable and integrated nucleic acid amplification microfluidic chip for identifying bacteria

Lab Chip. 2012 Apr 21;12(8):1495-9. doi: 10.1039/c2lc40055c. Epub 2012 Mar 7.

Abstract

In this work, we developed a portable integrated microchip of loop-mediated isothermal nucleic acid amplification (LAMP). This chip, with sample-to-answer capability, could perform rapid DNA release, exponential signal amplification and naked-eye result read-out in single or multiplex format. We call it iμLAMP, namely integrated micro-LAMP, which was successfully used for point-of-care identification of bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics*
  • Bacteria / isolation & purification*
  • Bacterial Infections / diagnosis
  • Base Sequence
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / isolation & purification
  • Equipment Design
  • Humans
  • Microfluidic Analytical Techniques*
  • Molecular Sequence Data
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification
  • Nucleic Acid Amplification Techniques / instrumentation*
  • Point-of-Care Systems*

Substances

  • DNA, Bacterial