Fluorescence turn-on detection of hypochlorous acid via HOCl-promoted dihydrofluorescein-ether oxidation and its application in vivo

Chem Commun (Camb). 2012 May 16;48(39):4677-9. doi: 10.1039/c2cc30265a. Epub 2012 Mar 2.

Abstract

We report three new water-soluble dihydrofluorescein-ether probes FCN1, FCN2 and FCN3 for the detection of hypochlorous acid (HOCl), which were designed on the basis of a specific HOCl-promoted oxidation reaction. This work also provided a useful method to monitor the accumulated HOCl in specific organelles using a zebrafish model.

MeSH terms

  • Animals
  • Brain Chemistry
  • Ether / chemistry*
  • Fluoresceins / chemistry*
  • Fluorescent Dyes / chemistry*
  • Hypochlorous Acid / analysis*
  • Hypochlorous Acid / chemistry
  • Intestines / chemistry
  • Mice
  • NIH 3T3 Cells
  • Oxidation-Reduction
  • Solubility
  • Water / chemistry
  • Zebrafish / embryology

Substances

  • Fluoresceins
  • Fluorescent Dyes
  • dihydrofluorescein
  • Water
  • Ether
  • Hypochlorous Acid