Detection of a possible bioterrorism agent, Francisella sp., in a clinical specimen by use of next-generation direct DNA sequencing

Makoto Kuroda; Tsuyoshi Sekizuka; Fumiaki Shinya; Fumihiko Takeuchi; Takayuki Kanno; Tetsutaro Sata; Shigeyuki Asano

doi:10.1128/JCM.06715-11

Detection of a possible bioterrorism agent, Francisella sp., in a clinical specimen by use of next-generation direct DNA sequencing

J Clin Microbiol. 2012 May;50(5):1810-2. doi: 10.1128/JCM.06715-11. Epub 2012 Feb 15.

Authors

Makoto Kuroda¹, Tsuyoshi Sekizuka, Fumiaki Shinya, Fumihiko Takeuchi, Takayuki Kanno, Tetsutaro Sata, Shigeyuki Asano

Affiliation

¹ Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan. makokuro@nih.go.jp

Abstract

Deep sequencing detected a potential bioterrorism agent, Francisella tularensis, in a human abscess sample (Iwaki-08) of unknown etiology. Identified single-nucleotide variations suggest that the Iwaki-08 case was associated with Francisella tularensis subsp. holarctica (biovar japonica) but not the highly virulent type A (Francisella tularensis subsp. tularensis).

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Abscess / microbiology*
Biological Warfare Agents
Francisella tularensis / classification*
Francisella tularensis / genetics
Francisella tularensis / isolation & purification*
High-Throughput Nucleotide Sequencing / methods*
Humans
Male
Middle Aged
Molecular Sequence Data
Sequence Analysis, DNA
Tularemia / diagnosis*
Tularemia / microbiology

Substances

Biological Warfare Agents

Associated data

GENBANK/DRA00044