Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay

Sara Henriksson; Mats Nilsson

doi:10.1007/978-1-61779-567-1_9

Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay

Methods Mol Biol. 2012:853:95-103. doi: 10.1007/978-1-61779-567-1_9.

Authors

Sara Henriksson¹, Mats Nilsson

Affiliation

¹ Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden.

PMID: 22323143
DOI: 10.1007/978-1-61779-567-1_9

Abstract

Padlock probes and rolling circle amplification are techniques which can be used for detection of DNA sequences in situ with high specificity and high signal to noise. The single-cell gel electrophoresis assay is used to measure DNA damage and repair in cells. Here, we describe how padlock probes and rolling circle amplification can be used to detect DNA sequences within comet preparations.

MeSH terms

Animals
Base Sequence
Comet Assay / methods*
DNA / chemistry
DNA / genetics*
DNA Damage
Gene Dosage
Humans
Molecular Sequence Data
Oligonucleotide Probes / chemistry
Oligonucleotide Probes / genetics*
Single-Cell Analysis / methods*

Substances

Oligonucleotide Probes
DNA