Abstract
The Fab from an IgG1, lambda murine monoclonal antibody with specificity for the O-polysaccharide antigen of Salmonella typhimurium has been crystallized in the absence and presence of hapten. The conditions for crystal growth were vapor diffusion equilibration with 16 to 23% polyethylene glycol 8000 solutions. Data have been collected from crystals of the complex in space group P212121, a = 60.6 A, b = 111.3 A, c = 61.1 A, and refinement of a molecular replacement solution is underway.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibody Specificity
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Antigens, Bacterial / chemistry*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Immunoglobulin Fab Fragments / immunology*
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Immunoglobulin G / immunology
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Molecular Sequence Data
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O Antigens*
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Polyisoprenyl Phosphate Sugars / chemistry*
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Polyisoprenyl Phosphate Sugars / immunology
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Salmonella typhimurium / immunology*
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X-Ray Diffraction
Substances
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Antibodies, Monoclonal
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Antigens, Bacterial
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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O Antigens
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O-specific polysaccharide, Salmonella
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Polyisoprenyl Phosphate Sugars