Covalent tagging of phosphorylated peptides by phosphate-specific deoxyribozymes

Amit Sachdeva; Madhavaiah Chandra; Jagadeeswaran Chandrasekar; Scott K Silverman

doi:10.1002/cbic.201200048

Covalent tagging of phosphorylated peptides by phosphate-specific deoxyribozymes

Chembiochem. 2012 Mar 19;13(5):654-7. doi: 10.1002/cbic.201200048. Epub 2012 Feb 7.

Authors

Amit Sachdeva¹, Madhavaiah Chandra, Jagadeeswaran Chandrasekar, Scott K Silverman

Affiliation

¹ Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

Abstract

Phosphopeptides tagged: Phosphorylated tyrosine and serine residues in peptides are modified selectively by DNA catalysts (see the figure). The deoxyribozymes catalyze covalent attachment of an RNA tag to a range of peptide sequences, establishing proof-of-principle for a new approach to phosphopeptide analysis.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA, Catalytic / metabolism*
Kinetics
Peptides / chemistry*
Peptides / metabolism*
Phosphates / metabolism*
Phosphorylation
Substrate Specificity

Substances

DNA, Catalytic
Peptides
Phosphates

Abstract

Publication types

MeSH terms

Substances

Grants and funding